Functional Characterization of Twin-arginine Translocation Gene(tatC) from Erwinia amylovora | 梨火疫病菌(Erwinia amylovora)双精氨酸运输系统基因(tatC)的功能分析
2011
Yu Yangyang, Nanjing Agriculture University, Nanjing(China), College of Plant Protection | Liu Qianqian, Nanjing Agriculture University, Nanjing(China), College of Plant Protection | Xu Enli, Nanjing Agriculture University, Nanjing(China), College of Plant Protection
Chinese. 梨火疫病菌(Erwinia amylovora)可引起梨、苹果等蔷薇科植物的火疫病。双精氨酸运输系统(Tat)与致病相关蛋白的转运有特定的联系。本研究在梨火疫病菌全基因组中发现了同源基因，通过同源重组的方法，构建了梨火疫病菌的tatC基因突变体以及互补子。研究结果表明，tatC基因影响着梨火疫病菌的致病性、胞外多糖、鞭毛运动、游动性、趋化性、生长情况等多种生物学特性。然而，EaΔtatC仍能引起烟草过敏性反应，并且在胞外纤维素和生物膜的生成方面与野生型菌株相比没有明显差异。说明梨火疫病菌tatC基因对病菌的生长、游动性以及致病性方面具有关键作用。
Show more [+] Less [-]English. Erwinia amylovora causes fire blight on many plants of the Rosaceae family such as apple and pear. The Twin-arginine translocation (Tat) pathway plays a crucial role in transporting the proteins which are related to virulence. In this study, a tatC disruption mutant EaΔtatC was successfully constructed by homologous recombination as well as the complement strain EaΔtatC (pME-tatC). The results showed that the tatC mutant EaΔtatC displayed the reduced virulence, extracellular polysaccharide production, chemotaxis, motility, flagella assembly and growth in vitro. However, compared to wild type strain NCPPB1665 (Ea1665), the induction of hypersensitive response in nonhost tobacco, biofilm synthesis and cellulose production of the tatC mutant EaΔtatC didn't show significant difference. These findings demonstrate that tatC gene in Erwinia amylovora is involved in growth and motility and play an important role in virulence.
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