Rapid flow cytometry method for triploidy determination in turbot (Scopthalmus maximus L.)

English. Flow cytometry was more recently applied to triploidy determination and usually carried out using erythrocytes.

English. High-quality histograms were obtained from turbot larvae and the very good reproductibility provided unambigous discrimination of diploids and triploids even in early stages development.

English. Accurate methods for assessing triploidy are important because treatments designed to induce poliploidy are not 100% and offspring from treated eggs are usually a mixture of triploids and diploids.

English. This paper describes a rapid and simple method for triploidy determination from the larval stage of turbot (Scopthalmus maximus) by flow cytometry.