Changes in the expression of CBL-genes in poplar genotypes of various morphological and systematic groups under abiotic stress S.G.

The study of the response to the impact of abiotic stress factors, conducted on genotypes of poplar belonging to different morphological and systematic groups is presented. Artificial modeling of salt and drought stress was performed. RNA was isolated from control and stress-affected samples, and then the expression of CBL1 and CBL2 genes was measured. It was found that the primers of transcription factors selected for the Populus trichocarpa species successfully amplify with other related poplar species and hybrids belonging to the same section and allow the expression level to be determined; however, for varieties and hybrids derived from white and black poplar, amplification gives a non-specific result. As a result, different responses of different poplar genotypes to drought and salinization were revealed. The analysis revealed a different response of individual poplar genotypes to drought and salinity. Changes in the expression of the CBL1 and CBL2 genes for the same genotypes also have a different character. Comparison of the response of the same genotypes to salinity and drought showed that a non-uniform result is observed for most of the samples. Different reactions of genes of the CBL family of selected genotypes in response to salt stress and drought, may be due to the genotypic features of varietal intrasectional and intersectional representatives. To identify general trends in the response of the considered genes to stress, needed further research, including the determination of changes in expression in dynamics.