The Association of Tanacetum parthenium and Salix alba Extracts Reduces Cortex Serotonin Turnover, in an Ex Vivo Experimental Model of Migraine
Simonetta Cristina Di Simone; Alessandra Acquaviva; Maria Loreta Libero; Annalisa Chiavaroli; Lucia Recinella; Sheila Leone; Luigi Brunetti; Matteo Politi; Claudia Giannone; Claudia Campana; Giustino Orlando; Gokhan Zengin; Massimo Tacchini; Luigi Menghini; Claudio Ferrante
The mixture of water extracts from Tanacetum parthenium and Salix alba was studied in an ex vivo assessment of neurotoxicity constituted by isolated mouse cortex specimens challenged with K+ 60 mM Krebs&ndash:Ringer buffer (neurotoxicity stimulus). The effects of the mixture on lactate dehydrogenase (LDH), nitrite and serotonin levels were investigated. The phytochemical profile of the mixture was also evaluated. A docking approach was conducted to predict, albeit partially, the putative mechanism underlying the observed effects. The extracts displayed a good profile of polyphenolic compounds (22 chromatographic peaks detected), with caftaric acid and epicatechin being the prominent phenols. In isolated cortex, the association of T. parthenium and S. alba extracts was effective in reducing the K+ 60 mM-induced levels of LDH and nitrites, whereas the neurotoxicity stimulus-induced serotonin depletion was prevented by the treatment. Regarding the inhibition of serotonin catabolism, epicatechin (44.65 µ:g/mg) and caftaric acid (10.51 µ:g/mg) were putatively the main compounds involved in the inhibition of monoamineoxidase-A, which is known to play a master role in serotonin turnover. Collectively, the results of the present study point to the efficacy of the present extract mixture as an innovative pharmacological tool to prevent the onset of migraine.
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