The Enhancing Effects of 10% PBS Washout of Holocene Minerals on HuIFN-αN3 Inducing Capacity of NDV ZG1999HDS or Sendai virus (Cantell Strain)
Bratko Filipič; Lidija Gradišnik; Adriana Pereyra; Gordan Mršić; Marjan Andrašec; Hrvoje Mazija
Different strains of Newcastle disease viruses (NDV) or Sendai viruses (SV) are used to induce the production of human leukocyte multi subtype interferon-alpha (HuIFN-&alpha:N3). Their inducing capacity can be enhanced in different ways. One includes 10% PBS washout of Holocene minerals (HM). The presented study aims to compare the HuIFN-&alpha:N3 inducing capacity of NDV ZG1999HDS or SV (Cantell strain) strain in vitro, and to evaluate the enhancing effect of 10% PBS washouts of HM on both viruses. The NDV strains&rsquo: ZG1999HDS interferon inducing capacity (483.23 ±: 4.5 pg/mL) was similar to that of the SV (Cantell strain) (584.16 ±: 5.9 pg/mL). It was shown that the HuIFN-&alpha:N3 inducing capacity of the strain of NDV ZG1999HDS can be strongly enhanced with 10% PBS washout of HM to 3818.21 ±: 41.9 pg/mL and 4790.34 ±: 33.5 pg/mL with SV (Cantell strain), u. The RP-HPLC analyses of such HuIFN-&alpha:N3 induced with the strain of NDV ZG1999HDS show the difference to SV (Cantell strain) induced HuIFN-&alpha:N3 in the absence of subtype &alpha:14 and the lower level of the subtype &alpha:1. The possible ways of such enhancement were also studied and it was postulated that the Fe2+ ions from 10% PBS washouts of HM, while stimulating the reactive oxygen species (ROS) and nitric oxide (NO) formation, activate the transcription factor NF- &kappa:B and consequently the production of HuIFN-&alpha:N3.
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