Influence of Electric Field on Proliferation Activity of Human Dermal Fibroblasts
Almaz Kamalov; Mikhail Shishov; Natalia Smirnova; Vera Kodolova-Chukhontseva; Irina Dobrovol’skaya; Konstantin Kolbe; Andrei Didenko; Elena Ivan’kova; Vladimir Yudin; Pierfrancesco Morganti
In this work, an electrically conductive composite based on thermoplastic polyimide and graphene was obtained and used as a bioelectrode for electrical stimulation of human dermal fibroblasts. The values of the electrical conductivity of the obtained composite films varied from 10&minus:15 to 102 S/m with increasing graphene content (from 0 to 5.0 wt.%). The characteristics of ionic and electronic currents flowing through the matrix with the superposition of cyclic potentials ±: 100 mV were studied. The high stability of the composite was established during prolonged cycling (130 h) in an electric field with a frequency of 0.016 Hz. It was established that the composite films based on polyimide and graphene have good biocompatibility and are not toxic to fibroblast cells. It was shown that preliminary electrical stimulation increases the proliferative activity of human dermal fibroblasts in comparison with intact cells. It is revealed that an electric field with a strength E = 0.02&ndash:0.04 V/m applied to the polyimide films containing 0.5&ndash:3.0 wt.% of the graphene nanoparticles activates cellular processes (adhesion, proliferation).
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