Functional Analysis of PsARRO−1 in Root Development of Paeonia suffruticosa
Yuke Sun; Wenqian Shang; Junhui Yuan; Zheng Wang; Songlin He; Yinglong Song; Liyun Shi; Yuxiao Shen; Jie Ma; Yufeng Xu; Jiange Wang
The adventitious rooting&minus:related oxygenase (ARRO&minus:1) gene is specifically expressed in the early rooting stage and is considered a molecular marker of rooting. In this study, a PsARRO&minus:1 gene (GenBank accession number KJ620008) was identified from a pre&minus:constructed transcriptome database of root development of Paeonia suffruticosa under sandy loam cultivation. The expression was verified by RT&minus:qPCR, and it was found that the expression trend was consistent with the expression in the transcriptome database. The PsARRO&minus:1 gene was specifically highly expressed during the root primordium germination phase. In addition, the RT&minus:qPCR analysis indicated that the expression of PsARRO&minus:1 in roots was significantly higher than in stems and leaves, its peak expression in vitro was 5 days earlier than in soil, and its expression was higher than that of tree peony in soil. Subcellular localization analysis showed that PsARRO&minus:1 was localized in the plasma membrane. Moreover, the transient silent expression of the PsARRO&minus:1 gene was found in the roots of peony seedlings grown using VIGS technology. The root activity was significantly reduced after transient silencing of the expression of the PsARRO&minus:1 gene. These results indicate that PsARRO&minus:1 has a positive regulatory effect on tree peony root development.
Show more [+] Less [-]AGROVOC Keywords
Bibliographic information
This bibliographic record has been provided by Multidisciplinary Digital Publishing Institute
