Impact of Organic Acids Supplementation to Hardwood Spent Sulfite Liquor as Substrate for the Selection of Polyhydroxyalkanoates-Producing Organisms
2018
Diogo Queirós | Catarina Rangel | Paulo Costa Lemos | Simona Rossetti | Luísa Seuanes Serafim
The effectiveness of polyhydroxyalkanoates (PHAs) production process from a waste stream is determined by the selection of a suitable mixed microbial culture (MMC). In this work, a feedstock from the paper industry, hardwood spent sulfite liquor (HSSL), supplemented with short-chain organic acids (SCOAs) to simulate a fermented effluent, was used as substrate to enrich a MMC in PHA-storing microorganisms. A stable culture was quickly established, and during the accumulation step the selected MMC reached a maximum PHA content of 34.6% (3HB:3HV-76:24). The bacterial community was analyzed through FISH analysis. Bacteria belonging to the four main classes were identified: Betaproteobacteria (44.7 ±: 2.7%), Alphaproteobacteria (13.6 ±: 1.3%) and Gammaproteobacteria (2.40 ±: 1.1%) and Bacteroidetes (9.20 ±: 3.8%). Inside the Betaproteobacteria class, Acidovorax (71%) was the dominant genus.
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