Identification of a Fomitopsis pinicola from Xiaoxing’an Mountains and Optimization of Cellulase Activity
2024
Jing Sun | Hong Yang | Shangjie Ge-Zhang | Yujie Chi | Dawei Qi
Brown-rot fungi are large fungi that can decompose the cell walls of wood: they are notable for their secretion of diverse and complex enzymes that synergistically hydrolyze natural wood cellulose molecules. Fomitopsis pinicola (F. pinicola) is a brown-rot fungus of interest for its ability to break down the cellulose in wood efficiently. In this study, through a combination of rDNA-ITS analysis and morphological observation, the wood decay pathogen infecting Korean pine (Pinus koraiensis Siebold and Zucc.) was identified. Endoglucanase (CMCase) and &beta:-glucosidase were quantified using the DNS (3,5-Dinitrosalicylic acid) method, and the cellulase activity was optimized using a single-factor method and orthogonal test. The results revealed that the wood-decaying fungus NE1 identified was Fomitopsis pinicola with the ITS accession number OQ880566.1. The highest cellulase activity of the strain reached 116.94 U/mL under the condition of an initial pH of 6.0, lactose 15 g·:L&minus:1, KH2PO4 0.5 g·:L&minus:1, NH4NO3 15 g·:L&minus:1, MgSO4 0.5 g·:L&minus:1, VB1 0.4 g·:L&minus:1, inoculated two 5 mm fungal cakes in 80 mL medium volume cultured 28 °:C for 5 days. This laid a foundation for improving the degradation rate of cellulose and biotransformation research, as well as exploring the degradation of cellulose by brown rot fungi.
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