Genome-Based Mining of Carpatamides I–M and Their Candidate Biosynthetic Gene Cluster
2024
Shu-Mei Shen | Yun-Chang Xie | Li-Rong Tu | Miao-Er Wu | Yan-Min Wang | Chun-Hui Song | Yu-Hui Sun | Ming-He Luo
Chemically investigating the marine-derived Streptomyces parvus 1268 led to the isolation of a new compound of carpatamide I (1). Subsequent genomic analysis identified its candidate biosynthetic gene cluster ctd of approximately 44 kb. In order to obtain more carpatamide derivatives, we conducted the upregulation of Ctd14, which is a positive regulator, and obtained improvement of carpatamide I and four new compounds of carpatamides J&ndash:M (2&ndash:5). The structures of the aforementioned five new isolates were identified by a combination of ESI-HRMS as well as one-dimensional (1D) and two-dimensional (2D) spectral NMR datasets. Bioassay results showed that compounds 1&ndash:5 displayed anti-inflammatory activity and weak cytotoxicity against cell lines of A549, HT-29, and HepG2.
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