Post-Exercise Whey Protein Supplementation: Effects on IGF-1, Strength, and Body Composition in Pre-Menopausal Women, a Randomised Controlled Trial
2025
Marc Murray | Lara Vlietstra | Alyssa M. D. Best | Stacy T. Sims | James A. Loehr | Nancy J. Rehrer
Introduction: The purpose of this study was to evaluate effects of post-exercise protein supplementation with combined resistance and interval training on total insulin-like growth factor-1 (IGF-1) concentration, strength (3RM), and body composition (DXA) in untrained pre-menopausal women. Methods: Twenty-seven women (33.6 ±: 9.2 years, 69.4 ±: 12.4 kg, 25.5 ±: 3.7 kg/m2) were randomised into a control (CON) or moderate protein group (PRO) (3 g, 24 g, resp.) and completed twelve weeks of upper-body resistance (2×:/week) and high-intensity interval cycle training (3×:/week). Linear mixed-effects model analyses were conducted. Results: PRO had a greater daily protein intake (5.0 ±: 16.6 g, 20.5 ±: 13.9 g, CON, PRO, resp., p = 0.025), with no change in IGF-1 (&minus:6.0 ±: 27.7 µ:g/L, &minus:2.1 ±: 27.8 µ:g/L, CON, PRO, resp., p = 0.920). Total lean mass increased (0.84 ±: 0.80 kg, 0.56 ±: 1.4 kg, CON, PRO, resp., p = 0.009), and all strength measures increased in both groups (19&ndash:113%, p <: 0.05). Conclusions: Untrained women can increase strength and lean mass over twelve weeks of combined resistance and interval training. Post-exercise protein supplementation had little effect, despite increasing protein intake by ~20 g/day in the PRO group. IGF-1 was not associated with any outcome measure.
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