Modified Aequorin Shows Increased Bioluminescence Activity
2007
Prasher, Douglas C.
Aequorin belongs to a unique class of photoproteins that emit light upon the binding of certain metals, calcium being the principal intracellular activator. This 'reporting function' of the metal-binding is instantaneous and is very easy to quantitate experimentally. The project objective was to develop a variety of recombinant forms of aequorin so they can be employed as metal biosensors. Three calcium-binding sites of aequorin were modified to examine their roles in the calcium-dependent luminescence as well as potentially binding other metal ions. Aequorins having Site 2 substitutions unexpectedly produce more light than wild-type aequorin.... Calcium, Bioluminescence, Photoprotein, Mutagenesis
Show more [+] Less [-]AGROVOC Keywords
Bibliographic information
This bibliographic record has been provided by AVANO
