Photocatalytical reduction of disulphide bonds in peptides on Ag-loaded nano-TiO(2) for subsequent derivatization and determination
2011
Wang, Lijuan | Yan, Xiaowen | Xu, Chunlai | Xiao, Zhiliang | Yang, Limin | 杨利民 | Zhang, Bo | 张博 | Wang, Qiuquan | 王秋泉
National Natural Science Foundation of China[21035006, 20775062, 20535020]; National Basic Research Program (973)[2009CB421605]
Show more [+] Less [-]We reported an alternative strategy to reduce disulphide bonds in peptides with Ag-nanoparticle loaded nano-TiO(2) (Ag/TiO(2)) under UV irradiation. The feasibility of this strategy was adequately demonstrated using the model peptides oxidized glutathione, vasopressin and insulin, which contain various disulphide bonds, as well as by its application to the determination of Cd-induced phytochelatins in Phaeodactylum tricornutum.
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