Sugarcane tissue culture [Philippines]
1981
Barba, R.C. | Zamora, A.B. | Mallion, A.K.
Callus formation of Philippine-bred sugarcane varieties was observed on modified Murashige and Skoog's medium for sugarcane with 2,4-D ranging from 0.05-5.0 mg/l. Supplementing the media with protein extracts, coconut water and sucrose hastens callus formation. Nutritional requirements for differentiation of sugarcane tissue cultures consisted of the base nutrient medium and combinations of additives such as sugar, coconut water, yeast extract and banana. Callus successively transferred to callus medium (high 2,4-D, 5.0 mg/l) continued to form callus but showed reduced ability to differentiate shoots when transferred to a differentiation medium. Color variants were noted among the plantlets produced, but no definite 2,4-D level could be correlated with the appearance of color variants. Substitutes for 2,4-D in inducing callus formation namely indole acetic acid and mapthoxy acetic acid used singly in concentrations ranging from 2 to 10 mg/l, were found unsatisfactory. On varying light intensity, differentiation of cultured sugarcane callus was favored by 200 and 700 foot candles. Plantlets exposed to 400 up to 1000 foot candles has a profusely branched root system, a high percentage of dry matter, a more vigorous stand, and a higher percentage of survival when transplanted to the soil. Potting experiments on newly harvested plantlets from tissue culture showed that misted conditions were more favorable for plantlet survival than the ordinary greenhouse conditions or conditions provided by a moist chamber. Compost, compost and rice hull, and white sand were the three best potting media
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