A plant sampling procedure for acetylene reduction assay to detect rice varietal differences in N2 fixation stimulation ability [Philippines]
1986
Padre, A.T. | Ladha, J.K. | Punzalan, G.C.
A plant sampling procedure for measuring acetylene-reducing activity (ARA) by a modified acetylene reduction (AR) assay for 3 consecutive days at heading stage is described. The method is suitable for detecting rice varietal differences in their ability to stimulate N2 fixation. The effects of environmental and plant related factors in ARA and its frequency distribution were studied. Reducing the variability in tiller number within a variety reduced variability in root and shoot dry weights and ARA. Day to day variation in ARA was not significant in most trials for 3 consecutive days of measurements. There were no significant differences in ARA among plants sampled at 0800, 1100 and 1600 h of the same day, but a higher variability among 3 consecutive days of measurements was obtained when sampling was done at 1600 h. Two of the 6 varieties tested approximated a log normal distribution while the other exhibited a distribution closer to normal. The coefficient of variation among ARA means with a sample size of 6 in about 11%. Results of some field experiments to study ARA abilities of several rice varieties are also presented
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