Identification, genotypic variation and virulence of Philippine isolates of the Baculovirus of Oryctes rhinoceros L. [Philippines]
1989
Alfiler, A.R.R.
Twenty-two isolates of the Oryctes baculovirus were collected from diseased specimens in major coconut growing region in the Philippines. The different baculovirus isolates were characterized using restriction endonuclease analysis with the enzymes BamHI, EcoRI, and HindIII and were found to have only very slight differences in their restriction fragment electrophoresis profiles. The relative virulence of the 22 isolates was tested by determining the LD50 for larvae and adults of Oryctes rhinoceros. LD50 values for larvae ranged from 4.86 ppm to 33.30 ppm of virus-killed larvae in the food and for adults from 4.83 to 14.77 pikoliters of hemolymph. The dosage mortality responses revealed no statistically significant differences among the different baculovirus isolates with the exception of one isolate which had a statistically different biological activity level against third instar larvae of O. rhinoceros. On the basis of the restriction fragmentation profiles and virulence to third instar larvae and adults of O.rhinoceros, the different isolates were found to be extremely closely related to the standard strain, PV505
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