Transgenesis - breeding for multiple plant protection
2002
Data, K. | Baisakh, N. | Thet, K.M. (International Rice Research Inst., Los Banos, Laguna (Philippines). Plant Breeding, Genetics and Biochemistry Div.) Tu, J. | Arboleda, M. | Oliva, N. | Datta, S.K.
Here the authors describe the development of transgene-pyramided stable elite rice lines resistant to disease and insect pests by conventional crossing of two transgenic parental lines transformed independently with different genes. The Xa21 gene (resistance to bacterial blight), Bt fusion gene (for insect resistance), and chitinase gene (for tolerance to sheath blight) were combined in a single rice line by reciprocal crossing of two transgenic homozygous IR72 lines. F4 plant lines carrying all the genes of interest stably were identified using molecular methods. The identified lines, when exposed to infection caused by Xanthomonas oryzae pv. oryzae, showed resistance to bacterial blight. Neonate larval mortality rates of yellow stem borer (Scirpophaga incertulas) is an insect bioassay of the same identified lines were 100 percent. The identified line stacked with different genes to protect against yield loss showed high tolerance of sheath blight disease caused by Rhizoctonia solani
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