Shoot culture and plant regeneration in Benguet lily (Lilium philipinensis)
1999
Zamora, A.B. | Gruezo, S.S. (Philippines Univ. Los Banos, College, Laguna (Philippines). Inst. of Plant Breeding)
The study was conducted to develop tissue culture protocols for the propagation of Benguet lily (Lilium philippinensis). The protocols included workable procedures for disinfection for aseptic establishment of plant tissues to culture, for propagation in vitro, rooting in vitro and successful potting out of tissue culture-derived plants. Sterile plant cultures were obtained with a disinfection procedure involving pre-washing, soaking in an appropriate disinfectant and use of young protected shoot tip tissues. Using aseptic plants grown from disinfection experiments, the critical factors for subsequent in vitro propagation included different plant parts (i.e. nodes, internode, leaves and shoot tip) and a cytokinin-supplemented MS (Murashige and Skoog, 1962) culture medium. Basal sections from aseptic leaves yielded globular structures and plants on BAP-medium. Complete plantlets formed from excised shoot tips and nodes even in simple MS culture media. Stem elongation, akin to the development of flower stems, was observed. Aerial and basal bulblets were obtained in vitro during prolonged shoot culture (i.e. 3-month culture cycle). Plantlets were readily acclimatized and survived potting out to a moist potting mix of coconut coir dust and soil
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