Seasonal changes in glutamate dehydrogenase activities and amino acid content in green asparagus during spear growth
2001
Enriquez, F.G. (Department of Agriculture, Estancia, Mandaue City, 6014 Cebu (Philippines). Seed Quality Control Services-7) Matsui, T. | Bhowmilk, P.K. | Suzuki, H. | Kawada, K.
The seasonal changes in glutamate dehydrogenase (GDH) amination and deamination activities in the top and bottom portions of the spear of two asparagus cultivars `Welcome' and `E-414' grown in greenhouse condition from March to October were investigated. In the two cultivars and spear portions, GDH amination activity showed an increasing trend in July then declined remarkably from August to September, and started to increase in October. On the other hand, GDH deamination activity increased gradually with the temperature changes during growth of the spears. The GDH deamination activity in the top portion was significantly higher than the bottom portion. Of the two cultivars, GDH amination and deamination activities of `Welcome' were relatively higher than `E-414' in both portions although not significantly higher in the top portion than the bottom portion of the spear. Glutamine, glutamic acid, asparagine and aspartic acid contents fluctuated during the growing season with a higher content at higher temperature and lower content at room temperature. These results clearly demonstrate that temperature had a great influence on the rate of metabolizing enzymes and accumulation of amino acid in asparagus spears
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