Kakawate(Gliricidia sepium) jacq. Kunth ex. Walph coumarin as antitermitic and antimicrobial compound
2007
Rabena, A.R., Univ. of Northern Philippines, Vigan City, Ilocos Sur (Philippines)
Kakawate(Gliricidia sepium)Jacq. Kunth ex. Walph is a medium sized tree belonging to the family Leguminosae,which has a variety of uses and are planted as a fence, livestock feed and fuel wood. They cover the fountains of Ilocos and remaining vegetation during the dry months of the year, from January to May. Leaves were chopped and soaked separately in water and petroleum ether. A compound was isolated by TLC with a melting point at 64degC.The compound was identified as coumarin. Bioassay against termites(Macrocerotermes losbanosensis) manifested 95% mortality (4 hrs) and 100% (8 hrs) using 0.02 g/ml impregnated in filter paper in a Petri dish. However, the bioassay of crude water-soluble Gliricidia extract showed 75% mortality (24 hrs). The pure compound were tested on three microorganisms, Escherichia coli, Staphylococcus aureus and Trichophyton mentagrophytes. T. mentagrophytes showed an average antimicrobial index(AI) of 1.45 clotrimazole showed inhibitions of AI at 2.0. The other two micro-organisms were not inhibited.
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