Effectiveness of antagonistic formulation to control bacterial wilt of tomato in greenhouse
2007
Thaveechai, N.; Suthumma, K., Kasetsart Univ., 10900, Bangkok(Thailand). Dept of PLant Pathology | Suyama, K., Tokyo Univ. of Agriculture, 156-8502, Setagaya-Ku, Tokyo(Japan)Lab; of PLant Pathology
An effectiveness of antagonistic formulation made from Bacillus subtillus strain CH4(BS)amending with different subtrates and carriers was evaluated for the control of Ralstonia solanacearum strain T0-Ud3(RS),the causal agent of bacterial wilt of tomato(BWT),under laboratory and greenhouse conditions, five antagonistic formulation were developed by mixing BS at 1.8x10 sup 8 cfu/ml with rice bran and talcum powder(F1), steamed sorghum seeds(F2), F2 + rice bran(F3), sako pellets(F4) and F4+rice bran(F5). After storage for 3 months at room temperature, F1 formulation had the highest inhibition of RS and increment of BS population (1.6x10 sup 10 cfu/ml) tested on NA plate in laboratory. The F1 formulation was further investigated on the control of BWT under greenhouse at the Asian Institute of Technology, Pathum Thani province. Populations of BS and RS, % tomato survival and tomato yield were determined at 8 weeks after inoculation of RS. The F1 formulation provided tomato survival by 66% and tomato yield by 200 gm/plant whereas non-treated control showed tomato survival by 11% and yield by 10 gm/plant which were significantly different. At the end of 8 weeks post inoculation, the BS population was significantly increased from the initial of 7.3x10 sup 6 cfu/ml to 1.2x10 sup 3 cfu/ml. This study indicated that F1 formulation containing BS: rice bran: talcum powder at 1:2:2(v/w/w)is effective for controlling BWT harvesting in greenhouse which is promising for further evaluation in farmer's field.
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