Metagenomics of Plutella xylostella (L.): insight into the role of bacteria in increased metabolism of insecticides
2019
Mascareñas-Bautista, Ma.A. | Cleofe, M.A.S. | Valenciano, P.J. | Simangan, S.S.L.
The Diamondback moth (DBM), Plutella xylostella (L.), is considered the most destructive pest brassicaeous crops (e.g cabbage, pechay). It is also notorious for developing resistance against different classes of insecticides, including those derived from Bacilus thuringiensis Berliner. Besides endogenous gene expression of an organisms, previous studies have shown that the microbial ecology of an organism can contribute to the host's biological function and overall well-being (g.g. aphids and Buchnera aphidicola Munson et. al., Raftia pachyptila Jones and Proteobacteria). Additionaly, it has been determined that the predominant microbiota in the gut of DBM are Protreobacteria, implying roles in digestion and nutrient ansorption. In line with this, the main objective of the study is to determine the difference in microbial populations of DBM between resistant and susceptible strains upon insecticide exposure. However, determining the microbial ecology at a baseline level is of vital importance because this will serve as a basis for analysis of the developed strains. Thus, the microbial ecology of 1st and 4th generation from Mankayan, and the different larval stages of a2nd generation population from Buguias, will be analyzed through metagenomics.
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