Detection of the orchid mutant Dendrobium bicaudatum Reinw.ex Lindley with Related Amplified Polymorphic (Srap) Sequence
2023
Darmawati, I.A.P. | Fitriani, Y. | Wijana, G.
In addition to increasing the diversity of orchids through hybridization, it can also be done through mutations with chemical or physical mutagens. In a previous study, mutations were carried out in D. bicaudatum orchids by administering the mutagen Ethyl Methyl Sulfonate (EMS). Detection of ochid plant mutants can be carried out molecularly with Sequen-Related Amplified Polymorphism (SRAP) the amplification targets of these markers are the Open Reading Frames (ORFs) in the genome, with paired (forward and reverse) primers. The forward primer sequence used by Me3: TGAGTCCAAACCGAAT, Me4: TGAGTCCAAAACCGGACC and Em2: the reverse is GACTGCGTACGAATTTGC. Em3: GACTGCGTACGAATTGAC, Em4: GACTGCGTACGAATTTGA. Four of the 14 samples of D. bicaudatumwere explants with albino morphology. These four explants separated from ten other non-albino explants at a coefficient of 0.23. The other ten explants were grouped in various positions, not grouped according to the EMS treatment given or based on their morphology. The explant with the most similar genetic fingerprint profile was B8. Normal to B8.Chimera (coefficient 0.96). However, these two explants had separated from the non-mutant explants at a coefficient of 0.63. The polymorphism values of SRAP markers tested on D. bicaudatum mutant orchids were 100% for three pairs of markers, and 91% for one pair of markers (Me4-Em4). In Me4-Em4, of the 11 loci that were successfully read, 10 of them were polymorphic, and one other locus was monomorphic. PIC values for SRAP markers on D. bicaudatum ranged from 0.32 to 0.37.
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