Micropropagation of Pistacia vera ‘Kerman’ | 阿月浑子的组织培养和快速繁殖
2010
Yu Fei, Beijing University of Agriculture, Beijing (China), Department of Plant Science and Technology | Su Shuchai, Beijing Forestry University, Beijing (China), College of Forestry | Shen Man, Beijing University of Agriculture, Beijing (China), Department of Plant Science and Technology
[Objective]The aim was to commercialize micropropagation of pistachio and provide good foundations for varieties improvement. [Method] The seeds and stem-segments were used as explants in the tissue culture of Pistacia vera ‘Kerman’, tissue culture condition and medium and transplant matrix were researched for micropropagation of pistachio,[Result] Mediums of 1/2DKW and 1/2DKW+3.00 mg/L of 6-BA +0.05 mg/L of NAA were suitable medium for seed germination and axillary bud induction of pistachio; Proliferation coefficient was 3.6 on the medium of 1/2DKW +4.00 mg/L of 6-BA + 0.05 mg/L of IBA; Rooting rate was up to 75% on the medium of 1/2DKW + 5.00 mg/L of IBA + 1.00 mg/L of NAA; The suitable transplantation matrix was 3/4 sand + 1/4 vermiculite，and survival rate of plant with root was above 79%. [Conclusion] The pistachio (Kerman) tissue culture technology system was established.
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