Research on tissue culture of Pteris vittata L. | 凤尾蕨科植物蜈蚣草的组织培养

Chinese. [目的]对凤尾蕨科植物蜈蚣草的组织培养方法进行研究。[方法]以野外采集的叶芽为外植体，以1/2 MS + 3％蔗糖+ 0.7％琼脂为基本培养基，通过激素配比试验，筛选蜈蚣草愈伤组织诱导和继代培养的培养基配方。[结果]从外植体诱导出愈伤组织最佳培养基配方为1/2 MS + 3％蔗糖+ 0.7％琼脂 + 0.5 g/L PVP + 0.1 mg/L KT + 0.5 mg/L 2,4-D；从愈伤组织诱导出GGB和从GGB诱导出幼苗最佳培养基配方为1/2MS + 3％蔗糖 + 0.7％琼脂+ 0.5g/L PVP + 1.0 mg/L KT + 0.01 mg/L 2,4-D；另外，使用1/2 MS + 3％蔗糖+ 0.7％琼脂 + 0.5 g/L PVP + 0.5 mg/L 2,4-D做继代培养可以使愈伤组织持续增殖。[结论]研究直接使用野外材料组织培养，简化了试验步骤，是一种方便快速的蜈蚣草组培方法。

English. [Objective] The aim was to study the tissue culture of Pteris vittata L. which was a species of fern belonging to the genus Pteris. [Method] The collected leaf bud was used as explants, and the 1/2 MS + 3% sucrose + 0.7% agar was used as the basic medium, the phytohormone ratio testing was used to screen the medium formula for callus induction and subculture of P. vittata. [Result] The best medium formula for the callus induced from explants was: 1/2 MS + 3% sucrose + 0.7% agar + 0.5 g/L PVP + 0.1 mg/L KT + 0.5 mg/L 2, 4-D; the best medium formula for the GGB induced from callus and the seedlings induced from GGB was: 1/2MS + 3% sucrose + 0.7% agar + 0.5 g/L PVP + 1.0 mg/L KT + 0.01 mg/L 2,4-D; in addition, the use of 1/2 MS + 3% sucrose + 0.7% agar + 0.5 g/L PVP + 0.5 mg/L 2,4-D for the subculture could make the continued proliferation of callus. [Conclusion] The direct use of field material for tissue culture had simplified the test procedure, thus being a rapid tissue culture method for P. vittata.