Biochemical Mechanism of Chlorantraniliprole Resistance in the Diamondback Moth, Plutella xylostella Linnaeus

HU  Zhen-di, FENG  Xia, LIN  Qing-sheng, CHEN  Huan-yu, LI  Zhen-yu, YIN  Fei, LIANGPei  , GAO  Xi-wu
      1State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai&rsquo;an 271018, P.R.China
2Bio-Tech Research Center, Shandong Academy of Agricultural Sciences, Ji&rsquo;nan 250100, P.R.China
3Shandong Rice Research Institute, Ji&rsquo;nan 250100, P.R.China

Biochemical Mechanism of Chlorantraniliprole Resistance in the Diamondback Moth, Plutella xylostella Linnaeus

2014

HU Zhen-di, FENG Xia, LIN Qing-sheng, CHEN Huan-yu, LI Zhen-yu, YIN Fei, LIANGPei , GAO Xi-wu (1State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai’an 271018, P.R.China 2Bio-Tech Research Center, Shandong Academy of Agricultural Sciences, Ji’nan 250100, P.R.China 3Shandong Rice Research Institute, Ji’nan 250100, P.R.China)

The insecticide chlorantraniliprole exhibits good efficacy and plays an important role in controlling the diamondback moth, Plutella xylostella Linnaeus. However, resistance to chlorantraniliprole has been observed recently in some field populations. At present study, diamondback moths with resistance to chlorantraniliprole (resistant ratio (RR) was 82.18) for biochemical assays were selected. The assays were performed to determine potential resistance mechanisms. The results showed that the selected resistant moths (GDLZ-R) and susceptible moth could be synergized by known metabolic inhibitors such as piperonyl butoxide (PBO), triphenyl phosphate (TPP) and diethyl-maleate (DEM) at different levels (1.68-5.50-fold and 2.20-2.89-fold, respectively), and DEM showed the maximum synergism in both strains. In enzymes assays, a high level of glutathione-S-transferase (GST) was observed in the resistant moth, in contrast, moths that are susceptible to the insecticide had only 1/3 the GST activity of the resistant moths. The analysis of short-term exposure of chlorantraniliprole on biochemical response in the resistant strain also showed that GST activity was significantly elevated after exposure to a sub-lethal concentration of chlorantraniliprole (about 1/3 LC50, 12 mg L-1) 12 and 24 h, respectively. The results show that there is a strong correlation between the enzyme activity and resistance, and GST is likely the main detoxification mechanism responsible for resistance to chlorantraniliprole in P. xylostella L., cytochrome P450 monooxygenase (P450) and carboxy-lesterase (CarE) are involved in to some extent.

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