Myeloid zinc finger 1 (MZF1) is the most important transcriptional factor for porcine follistatin promoter
2015
SUN Ya-meng, WANG Liang, YANG Xiu-qin, ZHANG Dong-jie, LIU Di (1College of Plant Protection, Agricultural University of Hebei, Baoding 071001, P.R.China 2Nuclear Institute for Food and Agricuture, Tarnab, Peshawar 25000, Pakistan)
Follistatin (FS) is a secreted protein, which was originally isolated from porcine follicular fluid. Expression of follistatin is tightly regulated during porcine growth and development. To study the essential transcriptional regions of the porcine FS promoter, ten primer pairs were designed to amplify segments with different lengths of the FS promoter from –1 800 to +16 bp. The products were then inserted into the pGL3-basic vector to analyze the relative luciferase activity. The results showed that the most remarkable changes of promoter activity were observed between constructs (–302/+16 bp)-FS and (–180/+16 bp)-FS (P<0.01). Further research showed that the reconstructed reporter plasmid lacking myeloid zinc finger 1 (MZF1) binding sequence had significantly decreased luciferase activity (P<0.05). Furthermore, the FS protein expression was significantly increased in PK15 cells while the MZF1 was overexpressed, suggesting that the short sequence TCCCCACC (the recognition site of transcription factor MZF1) was the most important for FS transcription activation in the porcine.
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