ANALYSIS OF POLYMORPHISM OF BETA CASEIN OF SLOVAK PINZGAU CATTLE BY PCR-RFLP FOR ALLELS A1 AND A2
2009
MARTINA MILUCHOVÁ | ANNA TRAKOVICKÁ | M. GÁBOR
The work was oriented to identification of -casein gene polymorphism and analysis of genotype structure in population of Slovak Pinzgau cattle. The material involved 89 cattle. Bovine genomic DNA was isolated by fenol-chlorophorm deprotenization and ethanol precipitation and used in order to estimate - casein genotypes by means of PCR-RFLP method. The PCR products were digested with DdeI restriction enzyme. In the population included in the study there were homozygote genotype A1A1 (27 animals), heterozygote genotype A1A2 (46 animals) and homozygote genotype A2A2 (16 animals). In the total population of cattle heterozygotes A1A2 – 0.5168 were the most frequent, while homozygotes A2A2 – 0.1798 were the least frequent ones. This suggests a slight superiority of allele A1– 0.5618.
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