Antibacterial activity of Cuminum cyminum essential oil and Nisin on Streptococcus inaie in lab and fillets of rainbow trout
2013
L. Roomiani
The antibacterial activity of different concentrations of Cuminum cyminum essential oil (0, 0.005, 0.015,0.045, 0.135, 0.405%) and nisin (0, 0.125, 0.25, 0.75 μg/ml) was explored in lab and food model (filletsof rainbow trout) in controling Streptococcus iniae for 15 days at 8 ºC. The micro-dilution method wasused to determine MIC and MBC for nisin and the essential oil, so used for food model. Results showedthat MIC and MBC for essential oil and nisin were 0.015, 0.005% and 0.25, 0.125 μg/ml, respectively.For the duration of 15 days no statistical significance was recorded between the different concentrationsof nisin and essential oil and control treatment. Results showed that the bacterial growth was delayed indifferent samples being treated with nisin and essential oil from 3 to 6 days for the given compoundsand 6 days for nisin and essential oil in combination. The highest synergistic effect of nisin with essentialoil was found in 0.135 and 0.405% concentrations for essential oil and 0.75 μg/ml for nisin at 8 ºC.
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