Mapping of spike and grain using F3 and F4 families in Becher × Kavir cross in barly
2017
Robab Daghaghelh | Hossein Sabouri | Hossein Hosseini Moghaddm | Essa Jorjani | Hosseinali Fallahi
Barley is one of the world's grain is the most important corps after maize, wheat and rice.In order to spike and grain mapping in F<sub>3</sub> and F<sub>4</sub> families in Becher × Kavir cultivars cross in barlypopulation of 103 families with parents in a randomized complete block design with three replications 2014-2016 during two growing seasons. Assessment transgressive segregation was observed that the existence of different allele combinations in their parents. Linkage map were prepared using SSR, iPBS, IRAP and ISSR marker that it covered 640 cM of barley genome. The distance between two markers 9.69 cM were estimated. Composite interval mapping identified fifteen QTLs in F<sub>3</sub> and F<sub>4</sub> families. QTLs for awn length, grain diameter and grain length was located in both F<sub>3</sub> and F<sub>4</sub> families. For grain length and number of spikelets in the F<sub>4 </sub>families was identified the major effect QTL by R<sup>2</sup> 12.2 and 15, with the percentage LOD 3.62 and 2.9 in 3.5 and 2.5 cM distance markers. Length grain and grain diameter were linked and co-location iPBS2076-5 markers in the F<sub>3</sub> generation. co-location QTLs For awn length, number of spikelets, grain length and grain diameter identified. QTL identification characteristics of the controller can be effective in improving performance. It is expected to be followed by validation in various places and populations identified QTL in this study programs used in marker-assisted selection and accurate positioning.
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