Generation of three human induced pluripotent stem cell sublines (UCLAi004-A, UCLAi004-B, and UCLAi004-C) for reproductive science research
2021
Erica C. Pandolfi | Timothy J. Hunt | Sierra Goldsmith | Kellie Hurlbut | Sherman J. Silber | Amander T. Clark
Three induced pluripotent stem cell sublines (hiPSCs) were generated from human dermal human dermal fibroblasts (HDFs) derived from a human skin punch biopsy. The biopsy was donated from a woman with known infertility due to ovarian failure. The hiPSC sublines were created using Sendai virus vectors and were positive for markers of self-renewal including OCT4, NANOG, TRA-1-81 and SSEA-4. Pluripotency was verified using PluriTest analysis and in vitro differentiation using Taqman Real-Time PCR assays for somatic lineage markers. This participant’s monozygotic twin sister also donated a skin-punch biopsy, whose resulting hiPSC lines were published previously as a resource.
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