Effects of Various Process Parameters on the Production of γ-Linolenic Acid in Submerged Fermentation
2006
Syed U. Ahmed | Sudheer K. Singh | Ashok Pandey | Sanjit Kanjilal | Rachapudi B. N. Prasad
Studies were carried out on the fermentative production of γ-linolenic acid (GLA) using seven strains belonging to Mucorales. An oleaginous fungal strain, isolated from the Western Ghats of Kerala produced GLA at a level of 8 % (by mass), when grown in a complex medium containing glucose as the sole carbon source. Effects of different culture conditions were investigated in shake flasks. Maximum dry biomass and total GLA obtained were 48.4 g/L and 636 mg/L, respectively, in the culture cultivated at 30 °C and 200 rpm for 7 days. Among the organic nitrogen sources investigated, yeast extract, and combination of corn steep liquor and baker’s yeast in 1:1 ratio were useful for enhancing the GLA production and the effects were comparable.
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