Regulation Mechanism of Dopamine Receptor 1 in Low Temperature Response of <i>Marsupenaeus japonicus</i>
2023
Xianyun Ren | Xueqiong Bian | Huixin Shao | Shaoting Jia | Zhenxing Yu | Ping Liu | Jian Li | Jitao Li
Dopamine receptors (DARs) are important transmembrane receptors responsible for receiving extracellular signals in the DAR-mediated signaling pathway, and are involved in a variety of physiological functions. Herein, the D1 DAR gene from <i>Marsupenaeus japonicus</i> (<i>MjDAD1</i>) was identified and characterized. The protein encoded by <i>MjDAD1</i> has the typical structure and functional domains of the G-protein coupled receptor family. <i>MjDAD1</i> expression was significantly upregulated in the gills and hepatopancreas after low temperature stress. Moreover, double-stranded RNA-mediated silencing of <i>MjDAD1</i> significantly changed the levels of protein kinases (PKA and PKC), second messengers (cyclic AMP (cAMP), cyclic cGMP, calmodulin, and diacyl glycerol), and G-protein effectors (adenylate cyclase and phospholipase C). Furthermore, <i>MjDAD1</i> silencing increased the apoptosis rate of gill and hepatopancreas cells. Thus, following binding to their specific receptors, G-protein effectors are activated by <i>MjDAD1</i>, leading to DAD1-cAMP/PKA pathway-mediated regulation of caspase-dependent mitochondrial apoptosis. We suggest that MjDAD1 is indispensable for the environmental adaptation of <i>M. japonicus</i>.
Show more [+] Less [-]AGROVOC Keywords
Bibliographic information
This bibliographic record has been provided by Directory of Open Access Journals
