Melibiose–X-Gal–MacConkey Agar for Presumptive Differentiation of <i>Escherichia albertii</i> from <i>E. coli</i> and <i>Salmonella</i> from Poultry Meat
2023
Samuel D. Annor | Karla S. Salazar | Suresh D. Pillai | Chris R. Kerth | Jason J. Gill | Thomas M. Taylor
The bacterial foodborne enteropathogen <i>Escherichia albertii</i>, despite enjoying increased attention paid to its pathogenesis, global dissemination, and antimicrobial resistance capacity, remains difficult to identify from human foods. The primary objective of this study was to develop and test a selective and differential plating medium for the isolation of <i>E. albertii</i> from enteric pathogens commonly transmitted via fresh poultry meat, namely <i>E. coli</i> and <i>Salmonella enterica</i>. MacConkey agar supplemented with α-D-+-melibiose and the lactose analogue X-gal was prepared and used to differentially enumerate <i>E. albertii</i>, <i>Salmonella</i>, and <i>E. coli</i> from inoculated ground chicken meat. The medium, MXgMac agar, differentiated the inoculated pathogens with a greater degree of efficiency than did the previously developed <i>E. albertii</i>-selective medium xylose–rhamnose–melibiose (XRM) MacConkey agar, based on differential usage of the lactose analogue and melibiose. Chicken-derived feces and litter samples were subsequently tested using the medium and found not to contain <i>E. albertii</i> by 16S rRNA gene amplification. MXgMac agar facilitates improved differential recovery of <i>E. albertii</i> and other enteric pathogens from poultry meat versus other <i>E. albertii</i> selective/differential media.
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