The effect of methyljasmonate elicitor on tropan alkaloid production in hairy roots of Atropa belladonna

<span style="font-family: 'Times New Roman',serif; font-size: 12pt; mso-bidi-font-size: 14.0pt; mso-bidi-font-family: 'B Lotus';" lang="EN-GB">The parasympatholytic tropane alkaloids, Atropine and Scopolamine, accumulated in <em>Atropa</em> <em>belladonna</em>, are of great interest for the pharmaceutical industry.</span><span style="font-family: 'Times New Roman',serif; font-size: 12pt; mso-bidi-font-size: 14.0pt; mso-bidi-font-family: 'B Lotus';" lang="EN-GB">In this research, <em>Atropa</em> <em>belladonna</em> transformed hairy roots were produced by <em>Agrobacterium</em> <em>rhizogenes</em> strain A4 mediated transformation. Effects of</span><span style="font-family: 'Times New Roman',serif; font-size: 12pt; mso-bidi-font-size: 14.0pt; mso-bidi-font-family: 'B Lotus'; mso-ansi-language: EN-US;"> different concentrations </span><span style="font-family: 'Times New Roman',serif; font-size: 12pt; mso-bidi-font-size: 14.0pt; mso-bidi-font-family: 'B Lotus';" lang="EN-GB">of methyl jasmonate </span><span style="font-family: 'Times New Roman',serif; font-size: 12pt; mso-bidi-font-size: 14.0pt; mso-bidi-font-family: 'B Lotus'; mso-ansi-language: EN-US;">(0, 150 and 300 </span><span style="font-family: 'Times New Roman',serif; font-size: 12pt; mso-bidi-font-size: 14.0pt; mso-bidi-font-family: 'B Lotus';" lang="EN-GB">Micro molar) were investigated on production of Atropine and Scopolamine in 24 hours treatment. Then the contents of two tropane alkaloids, Atropine and Scopolamine, were assayed by HPLC method. The tropane alkaloids contents were shown to be significantly increased in transformed hairy roots. Results showed Atropine and Scopolamine content in transformed hairy roots reached respectively from 0.12 to 1.715 and 0.0195 to 0.43 of mg in 100 mg of dry weight. Methyl jasmonate dramatically enhanced both tropane alkaloids, Atropine and Scopolamine. Tropane alkaloids content increased in 300 Mµ concentration of methyl jasmonat in comparison with 150 Mµ concentration and control treatment. In conclusion, it is suggested that, hairy root can be used as a replacement of plants in further studies and for tropane alkaloids production in economical and commercial scales.</span>