Supplementation of Nodulisporium sp. KT29 induced by Vibrio harveyi as an immunostimulant for controlling vibriosis in vannamei white shrimp under marine culture system

ABSTRACT The purpose of this study is to evaluate the effectiveness of Nodulisporium sp. KT29 supplementation with various Vibrio harveyi induction in feed against vibriosis in vannamei white shrimp. The study design included KP (positive control without supplementation Nodulisporium sp. KT29 and infected with V. harveyi), KN (negative controls without Nodulisporium sp. KT29 and infected with physiological solution), NT (treatment of supplementation Nodulisporium sp KT29 20 mL/kg and infected with V. harveyi), NM (treatment of supplementation test Nodulisporium sp. KT29 induction of V. harveyi dead cell 20 mL/kg and infected V. harveyi), NH (treatment of supplementation Nodulisporium sp. KT29 induction of V. harveyi live cell 20 mL/kg and infected with V. harveyi). The study parameters included inhibition zone, resistance, immune responses, and hemolim glucose. The results showed Nodulisporium sp. KT29 with induction treatment raised antibacterial activity with best treatment of NM and NH (P<0.05). The results of V. harveyi infection resistance presented NM treatment of 20 mL/kg increase survival in vannamei shrimp reached 72.2% (P<0.05). In addition, the same treatment increase the immune response activity and decrease the activity of hemolim glucose. It could be concluded that providing NM 20 mL treatment boosted the resistance and the immune system in vaname shrimp to control vibriosis reared at the sea. Keywords: antibacterial, β-glucan, induced, Nodulisporium sp. KT29, Vibrio harveyi ABSTRAK Tujuan penelitian yaitu mengevaluasi efektivitas suplementasi Nodulisporium sp. KT29 dengan berbagai perlakuan induksi Vibrio harveyi dalam pakan terhadap pengendalian vibriosis pada udang vaname yang dibudidayakan di laut. Rancangan penelitian meliputi KP (kontrol positif tanpa suplementasi Nodulisporium sp. KT29 dan diinfeksi V. harveyi), KN (kontrol negatif tanpa Nodulisporium sp. KT29 dan diinfeksi larutan fisiologis), NT (perlakuan uji suplementasi Nodulisporium sp. KT29 20 mL/kg dan diinfeksi V. harveyi), NM (perlakuan uji suplementasi Nodulisporium sp. KT29 diinduksi sel mati V. harveyi 20 mL/kg dan diinfeksi V. harveyi), NH (perlakuan uji suplementasi Nodulisporium sp. KT29 induksi sel hidup V. harveyi 20 mL/kg dan diinfeksi V. harveyi). Parameter penelitian meliputi zona hambat, resistensi, respons imun, dan glukosa hemolim. Hasil penelitian menunjukkan Nodulisporium sp. KT29 dengan perlakuan induksi dapat meningkatkan aktivitas antibakteri dengan perlakuan terbaik NM dan NH (P<0.05). Hasil pengamatan resistensi infeksi V. harveyi menunjukkan perlakuan NM 20 mL/ kg dapat meningkatkan kelangsungan hidup pada udang vaname mencapai 72.2% (P<0.05). Perlakuan yang sama juga meningkatkan respons imun dan menurunkan aktivitas glukosa hemolim. Disimpulkan bahwa pemberian NM 20 mL dapat meningkatkan resistensi dan sistem imun udang vaname terhadap pengendalian vibriosis di laut. Kata kunci : antibakteri, β-glucan, induksi, Nodulisporium sp. KT29, Vibrio harveyi,