The metabolism of 3α, 7α, 12α-trihydroxy-5β-cholestan-26-oic acid into cholic: an enzyme assay using homogenates of human liver
1976
R F Hanson | H L Sharp | G C Williams
An enzyme assay was developed to measure the conversion of the bile acid precursor, 3α, 7α, 12α-trihydroxy-5β-cholestan-26-oic acid (THCA), into cholic acid using homogenates of human liver biopsies. The average rate of metabolism of THCA into cholic acid was found to be 3.9 ± 0.5 (± 1 SD) pmoles of cholic acid formed/mg liver/minute in twelve normal liver biopsies. This assay system can be used to determine if the syndrome of neonatal cholestasis associated with a metabolic block in the conversion of THCA into cholic acid is transmitted as a genetic trait.
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