High-throughput identification of calcium-regulated proteins across diverse proteomes
2024
Timothy M. Locke | Rose Fields | Hayden Gizinski | George M. Otto | Melissa J.S. MacEwen | Domnita-Valeria Rusnac | Peixian He | David M. Shechner | Chris D. McGann | Matthew D. Berg | Judit Villen | Yasemin Sancak | Devin K. Schweppe
Summary: Calcium ions play important roles in nearly every biological process, yet whole-proteome analysis of calcium effectors has been hindered by a lack of high-throughput, unbiased, and quantitative methods to identify protein-calcium engagement. To address this, we adapted protein thermostability assays in budding yeast, human cells, and mouse mitochondria. Based on calcium-dependent thermostability, we identified 2,884 putative calcium-regulated proteins across human, mouse, and yeast proteomes. These data revealed calcium engagement of signaling hubs and cellular processes, including metabolic enzymes and the spliceosome. Cross-species comparison of calcium-protein engagement and mutagenesis experiments identified residue-specific cation engagement, even within well-known EF-hand domains. Additionally, we found that the dienoyl-coenzyme A (CoA) reductase DECR1 binds calcium at physiologically relevant concentrations with substrate-specific affinity, suggesting direct calcium regulation of mitochondrial fatty acid oxidation. These discovery-based proteomic analyses of calcium effectors establish a key resource to dissect cation engagement and its mechanistic effects across multiple species and diverse biological processes.
Show more [+] Less [-]Bibliographic information
This bibliographic record has been provided by Directory of Open Access Journals