Association of interleukin-2 gene variants (positions +114 and −384) and susceptibility to brucellosis in Iranian population
2017
Sohrab Najafipour | Abdolreza karami | Manoochehr Rasouli | Mehdi Kalani | Seyed-Jalal-Aldin Ashraf-Mansoori | Ali Moravej
<em>Brucella</em> is an obligate intracellular gram negative bacterium and the causative agent of brucellosis. Interleukin-2 (IL-2) is a cytokine involved in cell-mediated immunity response secreted by activated T-cells and considered as the growth factor for T-cells. Previous reports have revealed that gene polymorphisms of cytokines can affect susceptibility to <em>Brucella</em> infection. The goal of this study was to investigate the relationship between IL-2 gene polymorphisms (positions +114 and −384) and susceptibility to brucellosis. A total of 173 brucellosis patients and 75 healthy animal husbandmen who had <em>Brucella</em> infected animals and consumed their contaminated dairy products, as control group, were included in this study. All participants were genotyped for IL-2 gene polymorphisms at positions +114 (G/T) and −384 (G/T), using polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP). The frequency of TT genotype at the position +114 was significantly higher in the controls, compared with the patients. But, there was no significant difference between the groups regarding TG and GG genotypes as well as T and G alleles. Furthermore, at position −384, the frequencies of G allele and GG genotype were higher in the controls compared with patients, however, they were not significantly different Additionally, TT/TT haplogenotype (+114/−384) was significantly higher in the controls, compared with the patients. Conclusively, it is suggested that the inheritance of TT genotype (position +114) and TT/TT haplogenotype (+114/−384) of IL-2 gene could be considered as one of the genetic factors responsible for resistance to brucellosis.
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