Prime-seq, efficient and powerful bulk RNA sequencing
2022
Aleksandar Janjic | Lucas E. Wange | Johannes W. Bagnoli | Johanna Geuder | Phong Nguyen | Daniel Richter | Beate Vieth | Binje Vick | Irmela Jeremias | Christoph Ziegenhain | Ines Hellmann | Wolfgang Enard
Abstract Cost-efficient library generation by early barcoding has been central in propelling single-cell RNA sequencing. Here, we optimize and validate prime-seq, an early barcoding bulk RNA-seq method. We show that it performs equivalently to TruSeq, a standard bulk RNA-seq method, but is fourfold more cost-efficient due to almost 50-fold cheaper library costs. We also validate a direct RNA isolation step, show that intronic reads are derived from RNA, and compare cost-efficiencies of available protocols. We conclude that prime-seq is currently one of the best options to set up an early barcoding bulk RNA-seq protocol from which many labs would profit.
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