Generation of a human induced pluripotent stem cell line (SIAISi011-A) from a 61-year-old Chinese Han healthy female donor
2021
Wenxin Zhang | Yishi Hua | Weihao Di | Jinghui Guo | Qiuting Dai | Jian Zhao | Bei Zhang | Ying Wang
Peripheral blood mononuclear cells (PBMC) were donated by a healthy 61-year old Chinese Han female. Human OKSM (OCT3/4, KLF4 SOX2, and c-MYC) transcription factors were used to reprogram her PBMCs with the non-integrating episomal vector system. Immunocytochemistry for pluripotency makers confirmed the pluripotency of transgene-free iPSCs and their ability to differentiate spontaneously three germ layers in vitro. The iPSC line displayed a normal karyotype. Our model offers the possibility to be used a control in pathological mechanism studies.
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