Isolation of Primary Leydig Cells from Murine Testis
2021
Jinlian Liang | Yan Tang | Hanhao Li | Jiaxin Mei | Zhen Cao | Huan Xia | Rufei Huang | Yan Yang | Yadong Huang
In males, Leydig cells are the primary source of testosterone, which is necessary for testis development, masculinization, and spermatogenesis. Leydig cells are a valuable cellular model for basic research; thus, it is important to develop an improved method for isolation and purification of Leydig cells from testes. The available methods for Leydig cell isolation have some drawbacks, including the need for sophisticated instruments, high cost, tediousness, and time consumption. Here, we describe an improved protocol for isolation of primary Leydig cells from testicular tissue by digestion with collagenase IV.
Show more [+] Less [-]Bibliographic information
This bibliographic record has been provided by Directory of Open Access Journals