The Diversity of Culture-Dependent Gram-Negative Rhizobacteria Associated with <i>Manihot esculenta</i> Crantz Plants Subjected to Water-Deficit Stress
2021
Tatiana Zapata | Diana Marcela Galindo | Alba Rocío Corrales-Ducuara | Iván Darío Ocampo-Ibáñez
There is a lack of studies on the root-associated bacterial microbiome of cassava plants. The identification and characterization of rhizobacteria can contribute to understanding the adaptation of the agriculturally important crop plants to abiotic stress. Rhizobacteria play a significant role in plants, as they can alleviate the drought stress by various mechanisms that enhance the plant growth under these stressor conditions. In this study, Gram-negative bacterial strains from the plant rhizosphere of cassava <i>Manihot esculenta</i> Crantz CIAT MCOL1734 variety subjected to water deprivation were isolated, characterized according to their morphological properties, and then identified by VITEK<sup>®</sup> 2. An increase in the diversity, abundance, and species richness of Gram-negative rhizobacterial community was found in cassava plants subjected to water-deficit stress. In total, 58 rhizobacterial strains were isolated from cassava plants. The identification process found that the bacteria belonged to 12 genera: <i>Achromobacter</i>, <i>Acinetobacter</i>, <i>Aeromonas</i>, <i>Buttiauxella</i>, <i>Cronobacter</i>, <i>Klebsiella</i>, <i>Ochrobactrum</i>, <i>Pluralibacter</i>, <i>Pseudomonas</i>, <i>Rhizobium</i>, <i>Serratia,</i> and <i>Sphingomonas.</i> Interestingly, <i>Pseudomonas luteola</i> and <i>Ocrhobactrum anthropi</i> were rhizobacteria isolated exclusively from plants submitted to drought conditions. The cassava roots constitute a great reservoir of Gram-negative bacteria with a remarkable potential for biotechnological application to improve the drought tolerance of plant crops under water-deficit conditions.
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