The Fml1-MHF complex suppresses inter-fork strand annealing in fission yeast
2019
Io Nam Wong | Jacqueline PS Neo | Judith Oehler | Sophie Schafhauser | Fekret Osman | Stephen B Carr | Matthew C Whitby
Previously we reported that a process called inter-fork strand annealing (IFSA) causes genomic deletions during the termination of DNA replication when an active replication fork converges on a collapsed fork (Morrow et al., 2017). We also identified the FANCM-related DNA helicase Fml1 as a potential suppressor of IFSA. Here, we confirm that Fml1 does indeed suppress IFSA, and show that this function depends on its catalytic activity and ability to interact with Mhf1-Mhf2 via its C-terminal domain. Finally, a plausible mechanism of IFSA suppression is demonstrated by the finding that Fml1 can catalyse regressed fork restoration in vitro.
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