Identification and evolutionary analysis of cotton DUR3 gene
2023
Yuanyong GONG | Lihua ZHAO | Fei YAN | Yichen SUN | Hui WANG | Laihua LIU
Plant DUR3 homologous protein is a high affinity urea transporter which belongs to the family of sodium/solute symporter family, and plays an important role in the active absorption of exogenous urea and redistribution of endogenous urea by plants. The purpose of this study was to clarify structure and evolution situation of cotton DUR3 gene. Based on bioinformatics methods, DUR3 genes were identified from Gossypium hirsutum and G. raimondii genomic sequences, and then the gene structure, transmembrane domain, motif location, as well as phylogenetic relationship, were systematically analyzed. The results were as follows: (1) Three DUR3 genes were identified from the A and D subgroup chromosomes of upland cotton and G. raimondii genomic sequences. These three cotton DUR3 homologous proteins, like other plant DUR3 homologous proteins, had 15 transmembrane domains and three highly conserved motifs with consistent positions. (2) The gene structure analysis showed that the number of exons of DUR3 genes in dicotyledons was significantly higher than that in monocotyledons, and so were the cotton DUR3 genes. (3) Phylogenetic analysis revealed that the amino acid sequences of different species were classified according to species kinship, and cotton clustered in one branch with dicotyledons. (4) The Ka/Ks values of orthologous and paralogous DUR3 genes were generally more than one, indicating that those genes mainly experienced positive selection among evolution. The results of this study provide a theoretical basis for further research on cotton DUR3 homologous protein.
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