In Vitro Germination and Organogenesis of Endangered Neo-Endemic Baltic Dunes Species <i>Linaria loeselii</i> Schweigg
2024
Lidia Banaszczyk | Michał D. Starke | Damian Szelbracikowski | Julia Ścibior | Małgorzata Kapusta
This study focuses on the endangered neo-endemic Baltic dunes species <i>Linaria loeselii</i> Schweigg. (Plantaginaceae), also known as <i>Linaria odora</i> (M. Bieb.). By utilizing in vitro cultures, we successfully germinated seeds collected in situ. Our method, which involved using media supplemented with 5 µmol/L 6-benzylaminopurine, led to the indirect regeneration of shoots after 60 days of culture in the dark, significantly increasing the number of progeny plants. Additionally, the medium supplemented with 2.85 μmol/L indole-3-acetic acid and 10.2 μmol/L paclobutrazol allowed rooting after 30 days of shoot fragments. This research provides a potential basis for developing <i>Linaria loeselii</i> introduction programs into the environment, thereby contributing to the conservation of this endangered species.
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