Optimization of alcohol extraction process and in vitro lipid-lowering effects of Puerariae lobata
2022
DONG Hong-ying | PANG Hui-na | XIAO Feng-qin | SHAO Shuai | YAN Ming-ming
<b>Objective:</b> This study focused on exploring the <i>in vitro</i> lipid-lowering efficacy of <i>Puerarin lobata</i> extract. <b>Methods:</b> The adsorption ratios of oil, cholesterol, sodium glycocholate and sodium taurocholate were evaluated by a multi index comprehensive scoring method to optimize the <i>P. lobata</i> alcohol extraction process using Box-Behnken response surface methodology with extraction time, extraction times and ethanol concentration as influencing factors. <i>In vitro</i> lipid-lowering activity was further validated by comparing the levels of TG and TC accumulation and the extent of intracellular lipid droplet accumulation in a model of human hepatoma (HepG2) fatty liver cells induced by palmitic acid (PA). <b>Results:</b> The optimal alcohol extraction process of <i>P. lobata</i> was 70% by volume of ethanol, 1 h of extraction time and 3 times of extraction time; <i>P. lobata</i> extract could obviously reduce the contents of TG and TC in cells and effectively reduce the intracellular lipid droplet aggregation. <b>Conclusion:</b> The optimized <i>P. lobata</i> extraction process was stable and reliable, manipulable, and showed good lipid-lowering activity <i>in vitro</i>.
Show more [+] Less [-]Bibliographic information
This bibliographic record has been provided by Directory of Open Access Journals