Genome-Wide Characterization Analysis of <i>CCT</i> Genes in <i>Raphanus sativus</i> and Their Potential Role in Flowering and Abiotic Stress Response
2022
Qunyun Tan | Zhengjin He | Shiyong Mei | Jifang Zhang
<i>CCT</i> genes play vital roles in flowering, plant growth, development, and response to abiotic stresses. Although they have been reported in many plants, the characterization and expression pattern of <i>CCT</i> genes is still limited in <i>R. sativus</i>. In this study, a total of 58 <i>CCT</i> genes were identified in <i>R. sativus</i>. Phylogenetic tree, gene structure, and conserved domains revealed that all <i>CCT</i> genes were classified into three groups: <i>COL</i>, <i>CMF</i>, and <i>PRR</i>. Genome-wide identification and evolutionary analysis showed that segmental duplication expanded the <i>CCT</i> gene families considerably, with the LF subgenome retaining more <i>CCT</i> genes. We observed strong purifying selection pressure for <i>CCT</i> genes. <i>RsCCT</i> genes showed tissue specificity, and some genes (such as <i>RsCCT22</i>, <i>RsCCT36</i>, <i>RsCCT42</i> and <i>RsCCT51</i>) were highly expressed in flowers. Promoter cis-elements and RNA-seq data analysis showed that <i>RsCCT</i> genes could play roles in controlling flowering through the photoperiodic pathway and vernalization pathway. The expression profiles of <i>RsCCT</i> genes under Cd, Cr, Pb, and heat and salt stresses revealed that many <i>RsCCT</i> genes could respond to one or more abiotic stresses. Our findings could provide essential information for further studies on the function of <i>RsCCT</i> genes.
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