Antibacterial In Vitro Properties of Silver Sulfadiazine in Combination with Tris-EDTA and N-Acetylcysteine Against <i>Pseudomonas aeruginosa</i> Isolates from Dogs with Suppurative Otitis
2025
Ioanna Papadogiannaki | Rosario Cerundolo | Jennifer Plant | Elizabeth Villiers | Jenny Littler | Anika Wisniewska | Panagiotis Sgardelis
<i>Pseudomonas aeruginosa</i> otitis is common in dogs and antibiotic-resistant strains are often isolated. We are unaware of reports evaluating the combination of silver sulfadiazine (SSD) with a biofilm disruptor solution containing Tris-EDTA (tromethamine-ethylenediaminetetraacetic acid) and N-acetylcysteine (Tris-NAC). Forty-eight <i>P. aeruginosa</i> strains from dogs with suppurative otitis were analysed using the agar well diffusion method. A volume of 70 μL of Tris-NAC, a water solution of 10% SSD and their combination in equal amount was pipetted into the designated wells. After incubation, the diameter of the inhibition zone was measured. A synergy experiment using the checkerboard assay was performed to look at any potential synergistic effects of SSD and Tris-NAC against only 10 randomly selected isolates of <i>P. aeruginosa</i>. The samples tested with Tris-NAC + 10% SSD solution, compared with the samples tested with 10% SSD alone, demonstrated significantly higher inhibition zones against <i>P. aeruginosa</i>, <i>p</i> < 0.00001. The checkerboard assay results showed an additive effect between the two compounds. The use of 10% SSD could be evaluated as a therapeutic tool against strains of <i>P. aeruginosa</i> if combined with Tris-NAC.
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