Cloning and sequence analysis of two calmodulin isoform cDNA from mulberry
2008
WANG Wei | WANG Xing-ke | WANG Sheng-peng | SHEN Xing-jia | ZHAO Wei-guo
In order to elucidate the roles of calmodulin in mulberry stress-resistance, cDNA library of Fengchisang seedlings was constructed using SMART (switching mechanism at 5′ end of RNA transcript) cDNA library construction technology. 3' RACE (rapid amplification of cDNA end) acquired two calmodulin cDNA sequences from the library. Both of the sequences contained the 450 bp open reading frame (ORF), 3′-untranslated region. Sequence alignment of the two cDNAs show that in the ORF region the nucleotide similarity was 86%, but the amino acid similarity was 98%. It demonstrated that they are two calmodulin genes, named MCaM-1, MCaM-2. The amino acid similarity of MCaM-1 with CaM7 of Arabidopsis and CaM4 of Daucus carota was 100%, which showed that the calmodulins are conserved in plants.
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